大孔树脂提取链霉菌4903菌株除草活性物质的研究

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Abstract

Isolation of allelochemicals is an important step to study allelopathy. Our previous work demonstrated that Streptomyces strain 4903 showed allelopathic effects on both plants and plant fungal pathogens. In this study four macroporous resins (X-5, NKA-II, AB-8 and HP-20) were used to collect herbicidal substances from fermentation broth of Streptomyces strain 4903. Among the four resins, AB-8 macroporous resin had the highest adsorption capacity for herbicidal compounds from the fermentation broth. The static saturation adsorption capacity was 7 times that of resin volume. The optimal eluant eluted herbicidal compounds of Streptomyces strain 4903 from AB-8 resin was 80% (v/v) ethanol–water. All herbicidal compounds of Streptomyces strain 4903 were able to be desorbed by 2-3 times that of its resin volume. The AB-8 resin can be used for extraction and purification of herbicidal compounds from fermentation broth of Streptomyces strain 4903.

Key words： macroporous adsorption resin herbicidal substances AB-8 resin Streptomyces strain 4903

Received: 25 August 2013

TQ424.3

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	Chen Min
	WANG Rui-long
	Zeng Ren-sen

Cite this article:

Chen Min,WANG Rui-long,Zeng Ren-sen. Study on extraction of herbicidal substances from Streptomyces sp. 4903 with macroporous resins[J]. , 2013, 32(4): 469-473.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2013/V32/I4/469

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