Interspecific association among 15 populations of arborous layer and 9 shrub layer of the secondary forestcommunity was analyzed by the method of variance ratio(VR), means of Chi-square test, the 2×2 contingency tableanalyses and percentage co-occutring in Maofengshan. In general, 15 populations of arborous layer showed insignificantnegative association in the 10 000 m² plot. The main populations (Cinnamomum porrectum. Euodia lepta, Scheffleraheptaphylla and Pithecellobium lucidum) had weak associations with most of the other populations and significant associationswith quiet few populations. 9 populations of shrub layer showed negative association totality However the relationship among Euodia lepta. Psychotria rubus, Rubus septemlobus and Desmos chinensis was significantly positive. The relationship between Schefflera heptaphylla and Psychotria rubus was significantly positive too, but Pithecellobium lucidum and other populationswere not significantly associated. This paper also compared the advantage and disadvantage between the general index ofmulti-interspecies association and 3 indexes of pair-interspecies association and discussed the basic methods for themanagement and sustainable development of secondary forest in the ecological forest demonstration area in Maofengshan.