Preliminary study on distributions and causes of Apr, Kmr or Cmr bacteria in indigenous soil
LI Wei1, ZHONG Hui-bin1, LIU Hua1, DONG Ming-ming1, Xia Tao1, Zhang Xin2, ZHOU Tian-hong2
1. The Affiliated School of South China Normal University, Guangzhou, 510630, China; 2. Department of Biotechnology, Jinan University, Guangzhou, 510632, China
To study the distributions and causes of antibiotic resistance bacteria in indigenous soil, the bacteria were investigated on tests for antibiotic resistance(Apr, Kmr and Cmr, respectively)from the different environment. The plasmids from antibiotic resistance bacteria were isolated and identified. The results showed that there is no significance difference between the control and living area in Apr and Kmr bacteria. No Cmr bacterium was detected from samples. The Apr and Kmr bacteria with plasmid were both 18.2% in control area. The Apr and Kmr bacteria with plasmid were 36.4% and 27.3%, respectively in living area. These results suggest that the distribution of antibiotic resistance bacteria is no significance increase in indigenous soil.
[7] Davison J.1999.Genetic exchange between bacteria in the environment[J].Plasmid,42(2):73-91.
[8] Disque-Kochem C,Battermann A,Stratz M.2001.Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates[J].Microbiol Res,156(2):159-168.
[9] Linton A H.1988 Plasmids in the environment[J].Schriftenr Ver Wasser Boden Lufthyg,78:197-224.
[10] Nwosu V C.2001.Antibiotic resistance with particular reference to soil microorganisms[J].Res Microbiol.,152(5):421-430.
[11] Pepper I L,Gentry T J,Newby D T.2002.The role of cell bioaugmentation and gene bioaugmentation in the remediation of co-contaminated soils[J].Environ Health Perspect.,110 Suppl 6:943-946.
