Photosynthetic bacteria release H2 during N2 fixation. H2 may be evolved either by nitrogenase or hydrogenase,and the activity of the two enzymes,in turn, is influenced by the various physiological conditions. Among those factors, organic carbon restrains the hydrogenase expression but promote the H2 production. On the other hand, ammonium restrains the nitrogenase activity but decreases the H2 production;the existance of oxygen makes both nitrgenase and hydrogenase inactive, which restrains H2 evolved reaction consequently.
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