Duzhong(Eucommia ulmoides)is important Chinese medicine, due to it's high price and short supply, many duplicates are found in market. As Traditional taxonomy is difficult to ensure correct identification genuine Eucommia ulmoides, to develop an ultimate and definitive means for origin identification of genuine, the chloroplast matK gene was amplified by PCR using a consensus primer set and its nucleotide sequence was determined by PCR direct sequencing. The characteristic analysis of Eucommia ulmoides matk sequence was generated using Clustal aligned matK sequences of Apocynaceae and some related taxa, Celastraceae, and some most closely related matK sequences. 1140 bp matK gene sequences of Eucommia ulmoides were determined. Among 67 matK sequences analyzed, 32bp were unique in Eucommia ulmoides, including a "GAC" indels. These characteristic nucleotide loci might be used to obtain species-specific RFLP pattern or to design species-specific primers to ultimate identify genuine Duzhong. Chloroplast matK gene sequences might be a good molecular marker to be used to identify traditional Chinese medical plants.