The factors which influence the absorption of copper by Candida lipolytica were investigated, including the characteristics of the strain and culture time, pH of water, copper concentration, the strain dosage and the adsorption time. The adsorption kinetics was also detected. The titration of biomass indicated that-OH and-PO43-might exist on the surface of the biomass. The optimum culture time, pH and dosage were 96 h, 4.0-6.0 and 25.0g·L-1(wet weight), respectively. After 2 hours of adsorption more than 86.5% copper was removed by Candida lipolytica when copper concentration was 20mg·L-1 and the dosage of strain was 25.0g·L-1(wet weight). When the copper concentrations were 5mg·L-1 and 10mg·L-1, the removal efficiency of copper reached up to 95%. The kinetics analysis of copper onto the Candida lipolytica revealed that the bioadsorption process of copper in determinated concentrations was in accordance with Freundlich isotherm. The analysis of IR spectrogram indicated that the apex of-OH shifted for 18 cm-1 to long wavenumber, the other apices did not change a lot.
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