Investigation was carried out on osmotic permeability of biomembrane and antioxidization characteristics of asparagus under PEG stress with the concentrations of 10%,20% and 30%.The result showed that the contents of free Pro and soluble sucrose increased significantly with the test time,and reached the maximum at the treatment of 20% PEG-6000.The activities of CAT,POD and SOD increased with the concentration of PEG-6000,moreover,the relative conductivity and MDA increased with the test time and the concentrations of PEG-6000.This indicated that Asparagus officinlis seedlings adapted to drought stress by adjusting osmotic substance,decreasing water potential and improving protective enzymes activity to improve antioxydization ability under drought stress.