Accurate measurement of leaf area is a foundation for evaluating energy flux, carbon flux, and water using of ecosystem. Current methods for measuring leaf area have various limitations and scope of use. Some of them are easy-to-use and accurate, but can only be used for small batches. It is very difficult for most methods to measure leaf areas in large batches in a relatively short period of time. In this study, we took Spartina alterniflora as an example to discuss a method based on scanner and dry-leaves weight, combined with Photoshop image processing technology, which can measure leaf areas instantly in large batches. Tested by a known power function relation between leaf length (y) and leaf pixel (x), ie. y=419.85x1.9693 (R≈1.00), our new method only yielded 3.651% (n=30) relative error. There were no significant difference between this method and a classic square method (P=0.473). It is concluded that this method can be used to measure leaf area in large batches in a relatively short period of time, and to measure various shapes of leaves with little change in the vein.
赵聪蛟, 邓自发, 周长芳, 陈琳, 刘静娜, 安树青*, 申瑞玲. 大批量测定植物(互花米草)叶面积的一种新方法[J]. , 2007, 26(4): 327-331.
ZHAO Cong-jiao, DENG Zi-fa, ZHOU Chang-fang, CHEN Lin, LIU Jing-na, AN Shu-qing, SHEN Rui-ling. A new method of instantly measuring leaf area in large batches:a case study of Spartina alterniflora. , 2007, 26(4): 327-331.
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