The pot experiment was conducted to study the inoculation effects of nitrogen-fixing bacteria(Phy),or/and phosphate-solubilizing bacteria(Vib) on the growth and nutrients uptake of Rhizophora stylosa seedlings.The growth indexes of seedlings were assessed 6 months after inoculation.Statistic analysis revealed that either Phy or Vib inoculation could improve the nutritional status,enhance photosynthesis,and stimulate the growth of seedlings(with the increase of height,ground diameter,and biomass).Dual inoculation of Phy and Vib yielded the highest plant dry weight and nutrient uptake compared to the single inoculation.Dual inoculation of Phy and Vib increased plant dry height,diameter,underground biomass,total biomass,total nitrogen of roots,total phosphorus of roots and total chlorophyll content of leaves by 43.3%,11.6%,44.8%,29.9%,29.3%,27.0% and 16.8% compared to the uninoculated plant,respectively.Dual inoculation shows some positive interaction on the growth of R.stylosa seedlings,and has great potential for inoculation at seedling stage.
李玫1, 何雪香2, 廖宝文1. 固氮菌与溶磷菌接种对红海榄生长的影响[J]. , 2008, 27(4): 222-226.
LI Mei1, HE Xue-xiang2, LIAO Bao-wen1. Inoculation of nitrogen-fixing bacteria and phosphate-solubilizing bacteria on the growth of Rhizophora stylosa. , 2008, 27(4): 222-226.
[5] Bashan Y,Holguin G.1997.Azospirillum-plant relationships:environmental and Physiological advances (1990-1996)[J].Can.J.Microbiol,43:103-121.
[6] Vazquez P,Holguin G,Puente M E,et al.1999.Phosphate-solublizing microorganisms associated with the rhizosphere of mangroves in a semiarid coastal lagoon[J].Biol Fertil Soils,8:125-146.
[7] Okon Y,Albrecht S L,Burris R H.I977.Methods for growing Spirillum Lipoferum and for counting it in pure culture and in association with plants[J].Appl.Environ.Microbiol.,33:85-88.
[14] Rojas A,Holguin G,Gliek B R,et al.2001.Synergism between Phyllobacterium sp.(N2-fixer) and Bacillus licheniformis (P-solubilizer),both from a semiarid mangrove rhizosphere[J].FEMS Microbiol.Ecol.,35:181-187.
[16] Ahmad F,Abroad I,Aqil F,et al.2006.Plant growth promoting potential of free-living diazotrophs and other rhizobacteria isolated from Northern Indian soil[J].Biotechnol.J,1:1112-1123.
