Proteomic study of andrographolide analogue mediated protection on cell apoptosis induced by H2O2 in RIN-m beta cells
ZHOU Hui-hua1, DING We1n, JIA Hong-ling1, WANG Yu-qiang2, YAN Guang-rong1
1. Institute of Life and Health Engineering, Jinan University, Guangzhou 510632, China; 2. Institute of New Drug Research, Jinan University College of Pharmacy, Guangzhou 510632, China
To screen the differential proteins invovled in andrographolide analogue (AL-1) against apoptosis induced by hedrogen peroxide (H2O2) in Rin-m beta cells and investigate its molecular mechanism with proteomics. The results showed that:The survival rate of cells treated by H2O2 were increased by AL-1 in a dose-dependent manner. With proteomics analysis, 18 proteins related to apoptosis, stress were successfully identified, such as Prohibitin, Shmt2, Rho GDP-dissociation inhibitor-1, Galectin-1, Cyt b5, Hsps, and so on. Among them, 9 proteins were found to be up-regulated and 9 down-regulated in the AL-1 treated group compared with control (H2O2) group. AL-1 protected Rin-m beta cells against apoptosis induced by H2O2 via regulating proteins associated with apoptosis, stress.
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