环境因子及超低温冻存对黄姑鱼精子活力的影响

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摘要

通过测定精子的激活率、运动时间及寿命研究了环境因子变化对黄姑鱼精子活力的影响及超低温冻存后黄姑鱼精子的活力。结果表明,黄姑鱼精子激活与运动的适宜盐度为25～35、适宜pH为7.5～8.5。在pH 8.0～8.5、盐度25条件下,精子激活率达(85.33±2.52)%,运动时间及寿命分别为(336±14.02)s及(405.33±12.22)s。精子激活与运动的适宜NaCl、KCl、MgCl₂及葡萄糖溶液浓度分别为300～500 mmol·L^-1、600 mmol·L^-1、800～1000 mmol·L^-1及900mmol·L^-1;精子在缺少HCO₃^-的人工海水中未能被激活;精子在无Ca²⁺或无Mg²⁺的人工海水中激活率均大于80%,但运动时间及寿命均有所缩短。以Cortland及HBSS溶液为稀释液、10%EG为抗冻剂冻存黄姑鱼精子,冻精激活率>80%,运动时间均超过200s。

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	闫家强
	魏平
	姜建湖
	李海燕
	竺俊全

关键词 ：黄姑鱼, 精子活力, 超低温保存

Abstract：

We investigated the effects of environmental factors and cryopreservation on the vitality of sperm in Nibea albiflora.The results indicated that the suitable salinity for sperm activation and movement was 25-35 while pH value 7.5-8.5.The highest activation rate,the longest moving time and life-span of sperm were(87.00±5.54)%,(336±14.02) s,(405.33±12.22) s,respectively when the sperm was activated in the solutions(salinity25,pH 8.0-8.5).Also the suitable concentration of KCl,NaCl,MgCl₂,glucose solutions for sperm activation was 300-500mmol·L^-1,600mmol·L^-1,800-1000 mmol·L^-1 and 900 mmol·L^-1,respectively.The sperm could not be activated by HCO₃^- free artificial seawater and more than 80% of sperms were activated by Ca²⁺-free,Mg²⁺-free artificial seawater while the moving time and life-span of sperm was shortened significantly.The activation rate and moving time of cryopreservated sperm exceeded 80% and 200s when Cortland and HBSS solutions were used as extender and 10%EG as cryoprotectant.

Key words： Nibea albiflora sperm motility cryopreservation environmental factors

收稿日期: 2010-08-25

S965.325

基金资助:宁波市科学技术局项目(2006C100044,2007A31004);宁波大学人才工程项目(BSL200900);宁波大学2009年研究生科研创新基金项目;教育部创新团队项目(IRTO734);

引用本文:

闫家强, 魏平, 姜建湖, 李海燕, 竺俊全*. 环境因子及超低温冻存对黄姑鱼精子活力的影响[J]. , 2010, 29(4): 339-344.
YAN Jia-qiang, WEI Ping, Jiang Jian-hu, LI Hai-yan, ZHU Jun-quan. Effects of environmental factors and cryopreservaytion on the vitality of sperm in Nibea albiflora. , 2010, 29(4): 339-344.

链接本文:

http://www.ecolsci.com/CN/Y2010/V29/I4/339

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