In the present study,genetic relationship and geographic distribution of Enteromorpha genus in China coast were clarified. The samples were collected from Zhanqiao of Qingdao,Qianggang of Yancheng,Xiangshan of Ningbo and Pingyang of Wenzhou.The fragments of ITS1,5.8S rDNA and ITS2 were amplified,cloned and sequenced.The length of genetic fragments of rbcL from these four regions were all 1201bp.The nucleotide diversity and genetic distance were analyzed,and phylogenetic trees were constructed with neighbor-joining method.The results showed that the rate of evolution of ITS was the fastest,while rbcL was quite conservative.The region of ITS was short,with the content of GC up to over 65%,however the content of GC in 5.8S rDNA was 50%.ITS1 was more diverse than ITS2.There were geographic variations among the samples from the four sites.Genetic relationship between samples from Qingdao and Yancheng was closer,and so was that between samples from Xiangshan and Wenzhou.Ulva and Enteromorpha did not separate into an independent branch respectively,but they clustered together.So it can be concluded that genetic relationship between Ulva and Enteromorpha is close.Either Enteromorpha linza or Enteromorpha prolifera from Yancheng may caused the green tide in Qingdao.
王亚军, 仪茜茜, 王钢, 孙雪, 杨锐*. 中国沿海浒苔属rbcL和ITS序列比较及系统进化分析[J]. , 2010, 29(6): 507-511.
WANG Ya-jun, YI Qian-qian, WANG Gang, SUN Xue, YANG Rui. Phylogenetic analysis of rbcL and ITS of Enteromorpha genus from Chinese coast. , 2010, 29(6): 507-511.
[1] 曾呈奎,张德瑞,张峻甫.中国经济海藻志[M].北京:科学出版社.1962.43-50.
[2] 徐承斌,杨同娥.浒苔在池塘养殖中的危害及防治技术[J].齐鲁渔业,2002,19(1):31.
[3] 王惠冲.紫菜养殖中绿藻的综合防治[J].水产养殖,1992,1:5.
[4] 梁宗英,林祥志,马牧,张静,闫晓波,刘涛.浒苔漂流聚集绿潮现象的初步分析[J].中国海洋大学学报,2008,38(4):601-604.
[5] 李大秋,贺双颜,杨倩,刘俊鹏,于风,贺明霞,胡传民.青岛海域浒苔来源与外海分布特征研究[J].环境保护,2008,16:45-46.
[6] 蒋兴伟,刘建强,邹斌,王其茂,曾韬,郭茂华,朱海天,邹亚荣,唐军武.浒苔灾害卫星遥感应急监视监测系统及其应用[J].海洋学报,2009,3(1):52-64.
[7] 王超,乔洪金,潘光华,张宝玉,牛建峰,王广策,孙松,周百成.青岛奥帆基地海域漂浮浒苔光合生理特点的研究[J].海洋科学,2008,32(8):13-15.
[8] 王艇,苏应娟,朱建明.叶绿体rbcL基因序列在植物系统学研究中的应用[J].武汉植物研究所,1999,17(增刊):8-14.
[9] 张晓雯,毛玉泽,庄志猛,柳淑芳,王清印,叶乃好.黄海绿潮浒苔的形态学观察及分子鉴定[J].中国水产科,2008,15(5):822-829.
[10] Jiang P,Wang J,Cui Y Li,Li Y Xun,Qin S.Molecular phylogenetic analysis of attached Ulvaceae species and free-floating Enteromorpha from Qingdao coasts in 2007[J].Chinese Journal of Oceanology and Limnology,2008,26(3):276-279.
[11] 应成琦,张婷,李信书,田千桃,霍元子,马家海,徐韧,王金辉,何培民.我国近海浒苔漂浮种类ITS与18SrDNA序列相似性分析[J].水产学报,2009,33(2):215-219.
[12] Shimada S,Hiraoka M,Nabata S,Lima M,Masuda M.Molecular phylogentic analyses of the Japanese Ulva and Enteromorpha (Ulvales,Ulvophyceae),with special reference to the free floating Ulva[J].Phycological Research,2003,51:99-108.
[13] Hayden H S,Blomster J,Maggs C A,Silva P C,Stanhope M J,Waaland J R.Linnaeus was fight all along:Ulva and Enteromorpha are not distinct genera[J].Eur.J.Phycol.,2003,38:277-2.
[14] Leskinen E,Pamilo P.Evolution of the ITS sequences of ribosomal DNA in Enteromorpha[J].Hereditas,1997,126:17-2.
[15] 刘永健,杨官品,管晓菁.胶州湾浮游植物rbcL基因分子遗传多样性研究[J].应用生态学报,2004,15(9):1626-1632.
[16] 孙雪,杨锐,刘必谦,裴鲁青.坛紫菜rbcS及rbcL-rbcS基因间隔区的序列分析[J].水产科学,2006,25(1):23-26.
