It had been reported that the identification of species was accurate by genomic fingerprinting, so the evolution and identification of a Chinese traditional medicine Cuora trifasciata was studied by 18S ribosomal RNA gene sequences. The nuclear 18S rRNA gene region of muscles of Cuora trifasciata was amplified by PCR using a consensus primer set, and part of nucleotide was sequenced. The results showed that the length of 18S rRNA gene is 678 bp and GC is more abundance than AT. The application of DNA sequencing technique in the identify of Chinese traditional medicine was discussed.
[7] Cheung K S,Kwan H S,But P P H,et al.1994.Pharmacognostical identification of American and Oriedtal ginseng roots by genomic fingerprinting using arbitrarily primed polymerase chain reaction(AP-PCR)[J].J Ethnopharmacol,42:67.
[8] Fushimi H,Komatsu K,Isobe M,et al.1996.A new approach for the identification of a Chinese traditional medicine,Chuanxiong by 18S ribosomal RNA gene sequences[J].Phytomedicine,3:387.
[9] Fushimi H,Komatsu K,Isobe M,et al.1996.18S ribosomal RNA gene sequences of three Panax species and the corresponding ginseng drugs[J].Biol Yharm Bull,19(11):1530.
[10] Shirota O,Watanabe A,Yamazaki M,et al.1998.Random amplified polymorphic DNA and restriction fragment length polymorphism analyses of Cannabia sativa[J].Natural Medicines,52(2):160.
[11] Yamazaki M,Sato A,Shimomura K,et al.1994.Genetic relationships among Glycyrrhiza plans determined by RAPD and RFLP analyses[J].Biol Pharm Bull,17(11):1529.
