Sequencing of the 5’terminal region of Ls-rDNA from Pinus massoniana and the phylogeny of gymnosperm based on that
ZHANG Qun1, SHI Su-Hua2, QU Liang-Hu2, H.T. Chang2
1. College of Life Science and Technology, Jinan University, Guangzhou 510632, China; 2. Department of Biology, Zhongshan University, Guangzhou 510275, China
The 302-nucleotide sequences of the 5' terminal region of Ls-rDNA from Pinus massoniana lamb was determined. The phylogenetic tree based on the data of Ls-rDNA suggested that Pinus was more closely related to Taxodium and Gnetum than to other gymnosperms, the 3 genera constituted a monophyletic group, while Ginkgo and Cycas formed another monophyletic group within Gymnosperms, which might represent the two evolutionary procession of gymnosperms; The monophyly of both Gymnosperms and Angiosperms was supported. The potential of analysis of phylogeny of partial sequences of 5' terminal region of Ls-rDNA of higher level of taxon of seed plants was addressed.
章群1, 施苏华2, 屈良鹄2, 张宏达2. 马尾松Ls-rDNA5’末端序列分析及其系统学意义[J]. , 2003, 22(1): 27-29.
ZHANG Qun1, SHI Su-Hua2, QU Liang-Hu2, H.T. Chang2. Sequencing of the 5’terminal region of Ls-rDNA from Pinus massoniana and the phylogeny of gymnosperm based on that. , 2003, 22(1): 27-29.
[2] Aaron L,William A R.,James M O.1996.Length variation in the nuclear ribosomal DNA internal transcribed spacer region of the non-flowering seed plants[J].SysternaticBotany,21(2):109-120.
[3] Chaw,S M,H M Sung,H Long,et al.1994.Phylogeny of the major subclasses of angiosperms and data of the monocot-dic ot divergence[J].Am.J.Bot,81:S146.
[4] Peter R.Crane,Else M.Friis & Kaj R.Pedersen.1995.The origin and early diversification of angiosperms[J].Nature,374(2):27-33.
[5] Shi Su-hua,Qu Liang-hu,Xi Bao-qing,et al.1994.Sequenci ng and comparative analysis of the large-subunit ribosomal RNA 5' terminal region for four species of gymnosperms and their phylogenetic significance[J].Cathaya,6:35-42.
[6] Vadim Goremykin,Vera Bobrova,Jens Pahnke,et al.1996.Noncoding sequences from the slowly evolving chloroplast inverted repeat in addition to rbcL data do not support Gnetalean affinities of angiosperm[J].Mol.Biol.Evol,13(2):383-396.
