Cr3+,Cr6+及其复合污染对狐尾藻的毒害作用

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摘要

采用急性毒害水培法,研究比较了不同浓度的Cr⁶⁺,Cr³⁺单一及复合处理对狐尾藻不定根和叶片生理生态的影响。实验结果表明:1)当浓度≤1mg·L^-1时,Cr³⁺对不定根的生长表现出促进作用,数量增加,长度增长。而Cr⁶⁺及复合处理系列对不定根却表现出强烈的抑制作用,在高浓度时甚至使其生长停止;2)叶绿素含量在1mg·L^-1Cr³⁺培养液中达到最大值,随Cr⁶⁺及复合处理浓度的增大而呈连续下降趋势;3)可溶性蛋白含量在三种处理方式中都表现出抛物线形式,复合处理组在1mg·L^-1时使蛋白含量出现高峰;4)细胞内超氧阴离子产生速率,膜脂过氧化水平与污染浓度和时间呈显著正相关;5)随着处理浓度的增加,SOD,OD,CAT活性先升后降,但其高峰分别出现在处理浓度为1mg·L^-1,10mg·L^-1,5mg·L^-1时;6)研究证实,Cr⁶⁺,Cr³⁺复合处理对狐尾藻的毒害作用较单一Cr⁶⁺,Cr³⁺显著,二者表现出协同作用,而其中Cr⁶⁺毒性大于Cr³⁺。

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	胡韧
	林秋奇
	张小兰

关键词 ：重金属, 狐尾藻, 铬, 毒害

Abstract：

The paper briefly dealt with the toxic effects of Cr⁶⁺, Cr³⁺ and their combined pollution on the physiology of roots and leaves of high aquatic plant—Myriopllum verticillatum. The results show that Cr³⁺ could stimulate the growth of root when the treated concentration below 1 mg·L^-1, while Cr⁶⁺ and the combination treatment show significant inhibition to the growth of root. The highest concentration of chlorophyll gained when treated with Cr³⁺ at concentration of 1 mg·L^-1. The concentration of chlorophyll decreased as the increase of Cr⁶⁺ concentration. The concentration of soluble protein increased at first and decreased finally as the promotion of concentration of Cr⁶⁺, Cr³⁺ and their combined treatment. The same effects also took place on the activities of SOD, POD and CAT. The peak of concentration of protein occurred at the combination treatment of 1 mg·L^-1. The results suggest Cr⁶⁺ and Cr³⁺ have corroborative pollution effect to the M. verticillatum, and Cr⁶⁺ is more poisonous than the Cr³⁺.

Key words： Heavy metal Myriophyllum verticillatum Cr Toxic effect

收稿日期: 2003-08-25

:	Q949.751.9
	Q945.1
	Q614.33

基金资助:国家自然科学基金(39770046)

引用本文:

胡韧1, 林秋奇1, 张小兰2*. Cr3+,Cr6+及其复合污染对狐尾藻的毒害作用[J]. , 2003, 22(4): 327-331.
HU Ren1, LIN Qiu-qi1, ZHANG Xiao-lan2. Toxic effects of Cr3+, Cr6+ and their combined pollution on Myriophyllum verticillatum. , 2003, 22(4): 327-331.

链接本文:

http://www.ecolsci.com/CN/Y2003/V22/I4/327

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